Apr 29, 2003 · Protein expression was carried out at room temperature in a 96-well-plate format with buffered glycerol-complex medium (BMGY) consisting of 1% yeast extract, 2% peptone, 100 mM potassium phosphate buffer (pH 6.0), 1.34% yeast nitrogen base, 4 × 10 −5 % biotin, and 1% glycerol as a growth medium. The induction medium was buffered methanol
BMGY-Buffered Glycerol Complex Medium is used for culture of Pichia.. Temperature sensitive items must be shipped overnight. Name BMGY-Buffered Glycerol-Complex Medium Kit Product number B8000 1.2 Relevant identified uses of the substance or mixture and uses advised against Relevant identified uses General use 1.3 Details of the supplier of the safety data sheet Teknova 2290 Bert Dr. Hollister California 95023 United States Telephone: 831-637-1100 Telefax: 831-637-2355 buffered glycerol-complex medium (BMGY; 10 g/L of yeast extract, 20 g/L of peptone, 13.4 g/L of YNB, 4 £ 10¡4 g/L of biotin, 10 mL/L of glycerol and 0.1 mol/L of potassium phosphate buffer, pH 6.0) shaken at 250 rpm for 18 h at 30 C. The cell pellets were harvested and resuspended in 50 mL of buffered methanol-complex Mut s colonies (10) were then used to inoculate 10 ml buffered minimal glycerol-complex medium (BMGY) pH 6 (0. 1 M potassium phosphate buffer pH 6. 0, 1% yeast extract, 2% peptone, 1. 34% YNB without amino acids, 0. 4 μg ml −1 d-biotin, 1% glycerol) in a 50-ml loosely capped tube, shaken at 250 rpm for 2 days at 30°C, the cells were
Oct 29, 2010 · Mut S phenotype integrated into the grown colonies with the AOX1 locus of the yeast genome were selected and grown in 1 L of buffered glycerol complex medium to an optical density over 6 at 600 nm. Cells were induced with 1% methanol by replacing the buffered glycerol complex medium with buffered methanol complex medium.
Inoculates for the batch cultures were grown on a buffered glycerol-complex medium (BMGY; 10 g/L yeast extract, 20 g/L peptone, 100 mmol/L potassium phosphate, pH 6.0, 13.4 g/L YNB, 4×10-4 g/L biotin, 10 g/L glycerol).
In order to determine bacteriocin production kinetics, the clones P. pastoris X-33A, P. pastoris X-33B, and P. pastoris X-33AB were precultured in the buffered glycerol complex medium BMGY (10 g/liter yeast extract, 20 g/liter peptone, 100 mM potassium phosphate [pH 6], 1.34% yeast nitrogen base without amino acids, 4 × 10 −5 % biotin, 1%
Oct 26, 2019 · Strains were grown in 24‐well deep well plates (25°C, 600 rpm) using glycerol‐containing media (BMGY‐Buffered Glycerol Complex Medium; Teknova) supplemented to 4% (v/v) glycerol. After 24 hr of biomass accumulation, cells were pelleted and resuspended in either fresh BMGY or BMMY (Buffered Methanol Complex Medium; Teknova) containing 1.5 The applications of viral protein cages have expanded rapidly into the fields of bionanotechnology and materials science. However, the low-cost produc… A 1 mL aliquot was then used to inoculate a 1 L culture of buffered glycerol complex medium [1% yeast extract, 2% peptone, 100 mM potassium phosphate (pH 6), 1.34% yeast nitrogen base, 4 × 10 −5 % biotin, 1% glycerol]. Cells were grown at 25°C to an optical density of 10.